Journal: British Journal of Cancer

Article Title: Inhibition of the AURKA/YAP1 axis is a promising therapeutic option for overcoming cetuximab resistance in colorectal cancer stem cells

doi: 10.1038/s41416-024-02649-z

Figure Lengend Snippet: a Plot representing the distribution of YAP1 expression in CRC cell lines sensitive to cetuximab compared to the resistant ones ns =non-significant * p < 0.05, two-tailed t-Student’s test. b Plot depicting the distribution of YAP1 activity score in cetuximab-sensitive CRC cell lines compared to the resistant ones. c Correlation analysis between AURKA expression and YAP1 activity score. d Correlation analysis between AURKA and YAP1 expression levels. e Western blot illustrating the basal levels of total amount and phosphorylated YAP1 (Ser397) in CRC cell lines HCA46, SW48 and C10. Tubulin was used as a loading control. Results are plotted as the average ± SD of all the biological replicates and were normalised to the HCA46 cell line ( n = 3). ns =non-significant, ** p < 0.01, *** p < 0.001, one-way ANOVA. f Gene expression levels of CTGF and CYR61 in CRC cell lines HCA46, SW48 and C10 ( n = 3). ns =non-significant, ** p < 0.01, *** p < 0.001, one-way ANOVA.

Article Snippet: Human CRC cell line HCA46 was obtained from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Expressing, Two Tailed Test, Activity Assay, Western Blot, Control, Gene Expression

Journal: British Journal of Cancer

Article Title: Inhibition of the AURKA/YAP1 axis is a promising therapeutic option for overcoming cetuximab resistance in colorectal cancer stem cells

doi: 10.1038/s41416-024-02649-z

Figure Lengend Snippet: a Western blot illustrating c-MET levels in the HCA46, SW48 and C10 cell lines. Tubulin was used as a loading control. Results are plotted as the average ± SD of all the biological replicates and were normalized to the HCA46 cell line ( n = 3). b Colony formation assay of CRC cell lines HCA46, SW48 and C10.Results are plotted as the average ± SD of all the biological replicates. ** p < 0.01 ( n = 3), one-way ANOVA. c ALDH1 relative activity in CRC cell lines HCA46, SW48 and C10. Results were normalized to the cetuximab-sensitive HCA46 cell line. Results are plotted as the average ± SD of all the biological replicates. ** p < 0.01, *** p < 0.001, one-way ANOVA. d Western blot illustrating c-MET expression in SW48 and C10 cell line transduced with YAP1 and YAP1 S397A plasmids, along with the empty vector. Results were normalized to the YAP1-trasduced cell lines. e Colony formation assay of SW48 and C10 cell line transduced with YAP1 and YAP1 S397A plasmids, along with the empty vector. Results are plotted as the average ± SD of all the biological replicates, ** p < 0.01 ( n = 3), one-way ANOVA. f ALDH1 relative activity in SW48 and C10 cell line transduced with YAP1 and YAP1 S397A plasmids, along with the empty vector. Results were normalized to the empty vector condition in each cell line. Results are plotted as the average ± SD of all the biological replicates, * p < 0.05, ** p < 0.01 ( n = 3), one-way ANOVA. g SOX2 gene expression in SW48 and C10 cell line transduced with YAP1 and YAP1 S397A plasmids, along with the empty vector. Results were normalized to the empty vector condition in each cell line. Results are plotted as the average ± SD of all the biological replicates * p < 0.05, ** p < 0.01 ( n = 3), one-way ANOVA.

Article Snippet: Human CRC cell line HCA46 was obtained from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Western Blot, Control, Colony Assay, Activity Assay, Expressing, Transduction, Plasmid Preparation, Gene Expression